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mouse insulinoma cell line min6  (AddexBio Inc)

 
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    Structured Review

    AddexBio Inc mouse insulinoma cell line min6
    ( A ) Schematic design and 3D printer machine pathing G-code of a dual parallel channel multi-material CHIPS with pancreatic vascular cell bioink <t>(MIN6,</t> HUVEC, and MSC) regions surrounding both sides of the channels. ( B ) Pancreatic CHIPS FRESH printed and visualized via bright-field stereomicroscope (inset) and 3D confocal fluorescence imaging of the optically cleared pancreatic scaffold after 12 days of static culture. ( C ) XY midplane view from confocal fluorescence image of the 12-day statically cultured pancreatic CHIPS following 3D vascular network segmentation for quantification of network diameter and density within the migratory zones. ( D ) Example confocal fluorescence images revealing additional cell migration into the acellular regions of the CHIPS beneath the cellular regions guided by the printed collagen filaments. ( E ) XY midplane projection view from 3D confocal fluorescence imaging of the optically cleared 12-day VAPOR perfused pancreatic CHIPS. ( F ) Graphic illustration and example ROIs depicting evidence of early <t>MIN6</t> pancreatic bud and microlumen formation with actin (green) and insulin (magenta) fluorescence images from ROIs 2 and 3 in (E). ( G ) Graphic illustration and quantification for insulin secretion ELISA assay from 1.5-hour glucose-stimulated [ratio of high glucose (HG) to low glucose (LG)] insulin secretion experiment between 12-day static and perfusion cultured pancreatic CHIPS (means ± SD; ** P < 0.01 for N = 3 static tissues; N = 2 perfused tissues, unpaired t test).
    Mouse Insulinoma Cell Line Min6, supplied by AddexBio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse insulinoma cell line min6/product/AddexBio Inc
    Average 90 stars, based on 1 article reviews
    mouse insulinoma cell line min6 - by Bioz Stars, 2026-04
    90/100 stars

    Images

    1) Product Images from "3D bioprinting of collagen-based high-resolution internally perfusable scaffolds for engineering fully biologic tissue systems"

    Article Title: 3D bioprinting of collagen-based high-resolution internally perfusable scaffolds for engineering fully biologic tissue systems

    Journal: Science Advances

    doi: 10.1126/sciadv.adu5905

    ( A ) Schematic design and 3D printer machine pathing G-code of a dual parallel channel multi-material CHIPS with pancreatic vascular cell bioink (MIN6, HUVEC, and MSC) regions surrounding both sides of the channels. ( B ) Pancreatic CHIPS FRESH printed and visualized via bright-field stereomicroscope (inset) and 3D confocal fluorescence imaging of the optically cleared pancreatic scaffold after 12 days of static culture. ( C ) XY midplane view from confocal fluorescence image of the 12-day statically cultured pancreatic CHIPS following 3D vascular network segmentation for quantification of network diameter and density within the migratory zones. ( D ) Example confocal fluorescence images revealing additional cell migration into the acellular regions of the CHIPS beneath the cellular regions guided by the printed collagen filaments. ( E ) XY midplane projection view from 3D confocal fluorescence imaging of the optically cleared 12-day VAPOR perfused pancreatic CHIPS. ( F ) Graphic illustration and example ROIs depicting evidence of early MIN6 pancreatic bud and microlumen formation with actin (green) and insulin (magenta) fluorescence images from ROIs 2 and 3 in (E). ( G ) Graphic illustration and quantification for insulin secretion ELISA assay from 1.5-hour glucose-stimulated [ratio of high glucose (HG) to low glucose (LG)] insulin secretion experiment between 12-day static and perfusion cultured pancreatic CHIPS (means ± SD; ** P < 0.01 for N = 3 static tissues; N = 2 perfused tissues, unpaired t test).
    Figure Legend Snippet: ( A ) Schematic design and 3D printer machine pathing G-code of a dual parallel channel multi-material CHIPS with pancreatic vascular cell bioink (MIN6, HUVEC, and MSC) regions surrounding both sides of the channels. ( B ) Pancreatic CHIPS FRESH printed and visualized via bright-field stereomicroscope (inset) and 3D confocal fluorescence imaging of the optically cleared pancreatic scaffold after 12 days of static culture. ( C ) XY midplane view from confocal fluorescence image of the 12-day statically cultured pancreatic CHIPS following 3D vascular network segmentation for quantification of network diameter and density within the migratory zones. ( D ) Example confocal fluorescence images revealing additional cell migration into the acellular regions of the CHIPS beneath the cellular regions guided by the printed collagen filaments. ( E ) XY midplane projection view from 3D confocal fluorescence imaging of the optically cleared 12-day VAPOR perfused pancreatic CHIPS. ( F ) Graphic illustration and example ROIs depicting evidence of early MIN6 pancreatic bud and microlumen formation with actin (green) and insulin (magenta) fluorescence images from ROIs 2 and 3 in (E). ( G ) Graphic illustration and quantification for insulin secretion ELISA assay from 1.5-hour glucose-stimulated [ratio of high glucose (HG) to low glucose (LG)] insulin secretion experiment between 12-day static and perfusion cultured pancreatic CHIPS (means ± SD; ** P < 0.01 for N = 3 static tissues; N = 2 perfused tissues, unpaired t test).

    Techniques Used: Fluorescence, Imaging, Cell Culture, Migration, Enzyme-linked Immunosorbent Assay



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    ( A ) Schematic design and 3D printer machine pathing G-code of a dual parallel channel multi-material CHIPS with pancreatic vascular cell bioink <t>(MIN6,</t> HUVEC, and MSC) regions surrounding both sides of the channels. ( B ) Pancreatic CHIPS FRESH printed and visualized via bright-field stereomicroscope (inset) and 3D confocal fluorescence imaging of the optically cleared pancreatic scaffold after 12 days of static culture. ( C ) XY midplane view from confocal fluorescence image of the 12-day statically cultured pancreatic CHIPS following 3D vascular network segmentation for quantification of network diameter and density within the migratory zones. ( D ) Example confocal fluorescence images revealing additional cell migration into the acellular regions of the CHIPS beneath the cellular regions guided by the printed collagen filaments. ( E ) XY midplane projection view from 3D confocal fluorescence imaging of the optically cleared 12-day VAPOR perfused pancreatic CHIPS. ( F ) Graphic illustration and example ROIs depicting evidence of early <t>MIN6</t> pancreatic bud and microlumen formation with actin (green) and insulin (magenta) fluorescence images from ROIs 2 and 3 in (E). ( G ) Graphic illustration and quantification for insulin secretion ELISA assay from 1.5-hour glucose-stimulated [ratio of high glucose (HG) to low glucose (LG)] insulin secretion experiment between 12-day static and perfusion cultured pancreatic CHIPS (means ± SD; ** P < 0.01 for N = 3 static tissues; N = 2 perfused tissues, unpaired t test).
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    ( A ) Schematic design and 3D printer machine pathing G-code of a dual parallel channel multi-material CHIPS with pancreatic vascular cell bioink <t>(MIN6,</t> HUVEC, and MSC) regions surrounding both sides of the channels. ( B ) Pancreatic CHIPS FRESH printed and visualized via bright-field stereomicroscope (inset) and 3D confocal fluorescence imaging of the optically cleared pancreatic scaffold after 12 days of static culture. ( C ) XY midplane view from confocal fluorescence image of the 12-day statically cultured pancreatic CHIPS following 3D vascular network segmentation for quantification of network diameter and density within the migratory zones. ( D ) Example confocal fluorescence images revealing additional cell migration into the acellular regions of the CHIPS beneath the cellular regions guided by the printed collagen filaments. ( E ) XY midplane projection view from 3D confocal fluorescence imaging of the optically cleared 12-day VAPOR perfused pancreatic CHIPS. ( F ) Graphic illustration and example ROIs depicting evidence of early <t>MIN6</t> pancreatic bud and microlumen formation with actin (green) and insulin (magenta) fluorescence images from ROIs 2 and 3 in (E). ( G ) Graphic illustration and quantification for insulin secretion ELISA assay from 1.5-hour glucose-stimulated [ratio of high glucose (HG) to low glucose (LG)] insulin secretion experiment between 12-day static and perfusion cultured pancreatic CHIPS (means ± SD; ** P < 0.01 for N = 3 static tissues; N = 2 perfused tissues, unpaired t test).
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    min6 cells (mouse insulinoma cell line) - by Bioz Stars, 2026-04
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    Image Search Results


    ( A ) Schematic design and 3D printer machine pathing G-code of a dual parallel channel multi-material CHIPS with pancreatic vascular cell bioink (MIN6, HUVEC, and MSC) regions surrounding both sides of the channels. ( B ) Pancreatic CHIPS FRESH printed and visualized via bright-field stereomicroscope (inset) and 3D confocal fluorescence imaging of the optically cleared pancreatic scaffold after 12 days of static culture. ( C ) XY midplane view from confocal fluorescence image of the 12-day statically cultured pancreatic CHIPS following 3D vascular network segmentation for quantification of network diameter and density within the migratory zones. ( D ) Example confocal fluorescence images revealing additional cell migration into the acellular regions of the CHIPS beneath the cellular regions guided by the printed collagen filaments. ( E ) XY midplane projection view from 3D confocal fluorescence imaging of the optically cleared 12-day VAPOR perfused pancreatic CHIPS. ( F ) Graphic illustration and example ROIs depicting evidence of early MIN6 pancreatic bud and microlumen formation with actin (green) and insulin (magenta) fluorescence images from ROIs 2 and 3 in (E). ( G ) Graphic illustration and quantification for insulin secretion ELISA assay from 1.5-hour glucose-stimulated [ratio of high glucose (HG) to low glucose (LG)] insulin secretion experiment between 12-day static and perfusion cultured pancreatic CHIPS (means ± SD; ** P < 0.01 for N = 3 static tissues; N = 2 perfused tissues, unpaired t test).

    Journal: Science Advances

    Article Title: 3D bioprinting of collagen-based high-resolution internally perfusable scaffolds for engineering fully biologic tissue systems

    doi: 10.1126/sciadv.adu5905

    Figure Lengend Snippet: ( A ) Schematic design and 3D printer machine pathing G-code of a dual parallel channel multi-material CHIPS with pancreatic vascular cell bioink (MIN6, HUVEC, and MSC) regions surrounding both sides of the channels. ( B ) Pancreatic CHIPS FRESH printed and visualized via bright-field stereomicroscope (inset) and 3D confocal fluorescence imaging of the optically cleared pancreatic scaffold after 12 days of static culture. ( C ) XY midplane view from confocal fluorescence image of the 12-day statically cultured pancreatic CHIPS following 3D vascular network segmentation for quantification of network diameter and density within the migratory zones. ( D ) Example confocal fluorescence images revealing additional cell migration into the acellular regions of the CHIPS beneath the cellular regions guided by the printed collagen filaments. ( E ) XY midplane projection view from 3D confocal fluorescence imaging of the optically cleared 12-day VAPOR perfused pancreatic CHIPS. ( F ) Graphic illustration and example ROIs depicting evidence of early MIN6 pancreatic bud and microlumen formation with actin (green) and insulin (magenta) fluorescence images from ROIs 2 and 3 in (E). ( G ) Graphic illustration and quantification for insulin secretion ELISA assay from 1.5-hour glucose-stimulated [ratio of high glucose (HG) to low glucose (LG)] insulin secretion experiment between 12-day static and perfusion cultured pancreatic CHIPS (means ± SD; ** P < 0.01 for N = 3 static tissues; N = 2 perfused tissues, unpaired t test).

    Article Snippet: MIN6 (mouse insulinoma cell line) (Addexbio, C0018008) were cultured in high-glucose Dulbecco’s modified Eagle’s medium (DMEM, Gibco, 11-965-092) with 15% fetal bovine serum (v/v), 2 mM sodium pyruvate, 20 mM Hepes, and 0.05 mM β-mercaptoethanol, using passages 9 to 15.

    Techniques: Fluorescence, Imaging, Cell Culture, Migration, Enzyme-linked Immunosorbent Assay